Micro method for determination of borohydride with NAD+.
نویسندگان
چکیده
A spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD’ to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, there is a direct proportionality between the absorption at 340 nm and the amount of borohydride in solution over the range IO-100 nmoles, with an effective “molar extinction coefficient” of 12.2 X IO”. The method is simple, rapid, and sensitive.
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ورودعنوان ژورنال:
- Analytical biochemistry
دوره 54 2 شماره
صفحات -
تاریخ انتشار 1973